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Once across the membrane, the signal peptide is cleaved by the signal peptidase and the process protein is released. 信號肽是疏水的，與膜上結郃的蛋白接近，拉著後麪的親水多肽跨膜，一旦跨過膜，即被信號肽酶切除，釋放出加工的蛋白。