ECOR

常见例句

SV40T and FMDV-2A were ligated and cloned to the sites , sal I and EcoR I of pBGTKlox to make pBTGTKlox.
连接SV40T与FMDV-2A,回收后用EcoR I和Sal I酶切连接至pBGTKlox载体的相应位点,构成的新载体称为pBTGTKlox。
P16-promoter-luc-pGL2-Basic plasmid DNA was prepared after plasmid amplification, and digested by EcoR I and Hind III.
扩增p 16 promoter一luc一pGLZ一Basic报告基因表达质粒并提取其DNA。
Then the pEGF-6-his-tag fragment was cloned into plasmid pPIC9 at the sites of Xho I and EcoR I.
然后将PCR产物克隆到分泌表达载体pPIC9构建了pPIC9-pEGF。
The circle plasmid-like DNA pC1 was cloned into the EcoR site of pUC19 using E.coli JM109 as host.
将环形类质粒pC1连到pUC19的EcoR位点上，克隆至E.;coli JM109中。
The obtained bands were cloned into pGEM T-Easy vector, and the positive clone were confirmed by EcoR I enzyme digestion.
将所获片段克隆入pGEN T-Easy载体，EcoR I酶切鉴定。
In addition, the amplified IL-15 cDNA fragment from plasmid pBS-IL15 was digested with BamH I and cloned into EcoR I (filled in)/BamH I pBV220 vector.
另外，将经BamHI酶切的IL-15cDNA扩增片断与经EcoRI酶切、补平、再经BamHI酶切的pBV220载体相连，构建成重组质粒pBVB-IL15。